Effect of crowding by Dextrans in enzymatic reactions

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چکیده

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Effect of crowding by Dextrans in enzymatic reactions.

The interior of the living cell is highly concentrated and structured with molecules that have different shapes and sizes. Almost all experimental biochemical data have been obtained working in dilute solutions, situations which do not reflect the in vivo conditions. The consequences of such crowding upon enzymatic reactions remain unclear. In this paper, we have studied and compared the initia...

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The cell cytosol is crowded with macromolecules such as proteins, nucleic acids, and membranes. The consequences of such crowding remain unclear. How is the rate of a typical enzymatic reaction, involving a freely diffusing enzyme and substrate, affected by the presence of macromolecules of different sizes, shapes, and concentrations? Here, we mimic the cytosolic crowding in vitro, using dextra...

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ژورنال

عنوان ژورنال: Biophysical Chemistry

سال: 2014

ISSN: 0301-4622

DOI: 10.1016/j.bpc.2013.10.006