Effect of N-Acetyl-L-cysteine on Activity of Doxycycline against Biofilm-Forming Bacterial Strains

Biofilm-forming bacteria are associated with difficult-to-cure bacterial infections in veterinary patients. According to previous studies, N-acetyl-L-cysteine (NAC) showed an inhibitory effect on biofilm formation when it was applied combination beta-lactam antibiotics and fluoroquinolones. The lack of information about the NAC doxycycline activity against biofilm-forming strains reason for conducting this study. Staphylococcus aureus (S. aureus) ATCC 25923, O74, Escherichia coli (E. coli) 25922 Pseudomonas aeruginosa (P. aeruginosa) 27853 were used evaluate without addition planktonic formation. minimum concentrations (MICs) not affected by Gram-negative found be two times higher S. aureus. (MBICs) (2 μg/mL E. 32 P. 27853), determined using a standard safranin colorimetric assay, than MICs (0.5 4 μg/mL, respectively). data suggest that combinations could stimulate growth cells 25922. did affect strong