Effect of triptolide on estradiol release from cultured rat granulosa cells

Leptin modulates the corticotropin-releasing hormone (CRH) action on progesterone release from cultured rat granulosa cells.

OBJECTIVES: Corticotropin-releasing hormone (CRH) and leptin (LEP) are two neuropeptides involved in the regulation of food intake, energy homeostasis and stress response. These neuropeptides play an important role in the regulation of the hypothalamo-pituitary-gonadal (HPG) axis, too. It is known that leptin can affect the synthesis and release of CRH in the hypothalamus. There is no informati...

Effects of fenvalerate on steroidogenesis in cultured rat granulosa cells.

OBJECTIVE This study was designed to examine the in vitro effects of fenvalerate on steroid production and steroidogenic enzymes mRNA expression level in rat granulosa cells. METHODS Using primary cultured rat granulosa cells (rGCs) as model, fenvalerate of various concentrations (0, 1, 5, 25, 125, 625 micromol/L) was added to the medium for 24 h. In some cases, optimal concentrations of 22(R...

Inhibitory effects of the porcine follicular fluid on estradiol and progesterone secretion by cultured rat granulosa cells.

The effect of porcine follicular fluid on estradiol and progesterone secretion was examined using a rat granulosa cell culture with FSH and testosterone in the medium. Follicular fluids from small (less than 5 mm) and large (greater than 6 mm) follicles (SFFI, LFF1) were treated with charcoal, and then fractionated by filtration through an Amicon XM-50 and an PM-10 membrane. The addition of 25%...

Effects of prostaglandins on cultured granulosa cells from rhesus monkeys.

The Effects of Lithium Chloride on Survival and Function of in Vitro Cultured Human Granulosa Cells

Purpose: To investigate the effect of lithium chloride (LiCl) on survival and functional activity of in vitro cultured human granulosa cells (GCs). Materials and Methods: GCs were isolated from follicular fluid of women undergoing IVF/ICSI. After RBCs removal, GCs were plated in a medium containing varying concentrations of LiCl including 1, 2 and 5mM for two weeks period. A culture without Li...