Effects of Fibrillin-1 Degradation on Microfibril Ultrastructure
نویسندگان
چکیده
منابع مشابه
Fibrillin-1 microfibril deposition is dependent on fibronectin assembly.
Newly deposited microfibrils strongly colocalise with fibronectin in primary fibroblasts. Microfibril formation is grossly inhibited by fibronectin depletion, but rescued by supplementation with exogenous cellular fibronectin. As integrin receptors are key determinants of fibronectin assembly, we investigated whether they also influenced microfibril deposition. Analysis of beta1-integrin-recept...
متن کاملMicrofibril structure masks fibrillin-2 in postnatal tissues.
Fibrillin microfibrils are polymeric structures present in connective tissues. The importance of fibrillin microfibrils to connective tissue function has been demonstrated by the multiple genetic disorders caused by mutations in fibrillins and in microfibril-associated molecules. However, knowledge of microfibril structure is limited, largely due to their insolubility. Most previous studies hav...
متن کاملIdentification of a major microfibril-associated glycoprotein-1-binding domain in fibrillin-2.
Using yeast two-hybrid, ligand blotting, and solid phase binding assays, we have shown that microfibril-associated glycoprotein-1 (MAGP-1) interacts with the 8-cysteine motif of fibrillin-2 encoded by exon 24. Binding to this sequence was demonstrated for full-length MAGP-1 as well as for the MAGP-1 matrix-binding domain encoded by exons 7 and 8. The matrix-binding domain, but not the full-leng...
متن کاملFibrillin-1 Interactions with Heparin: Implications for Microfibril and Elastic Fibre Assembly *
Fibrillin-1 assembly into microfibrils and elastic fiber formation involve interactions with glycosaminoglycans. We have used BIAcore technology to investigate fibrillin-1 interactions with heparin, and with heparin saccharides that are analogous to S-domains of heparan sulfate. We have identified four high affinity heparin binding sites on fibrillin-1, localized three of these sites, and defin...
متن کاملFibrillin-1 interactions with heparin. Implications for microfibril and elastic fiber assembly.
Fibrillin-1 assembly into microfibrils and elastic fiber formation involves interactions with glycosaminoglycans. We have used BIAcore technology to investigate fibrillin-1 interactions with heparin and with heparin saccharides that are analogous to S-domains of heparan sulfate. We have identified four high affinity heparin-binding sites on fibrillin-1, localized three of these sites, and defin...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2007
ISSN: 0021-9258
DOI: 10.1074/jbc.m606370200