Effects of injecting calcium-buffer solution on [Ca2+]i in voltage-clamped snail neurons
نویسندگان
چکیده
منابع مشابه
The Effects of HCl and CaCl2 Injections on Intracellular Calcium and pH in Voltage-clamped Snail (Helix aspersa) Neurons
To investigate the mechanisms by which low intracellular pH influences calcium signaling, I have injected HCl, and in some experiments CaCl(2), into snail neurons while recording intracellular pH (pH(i)) and calcium concentration ([Ca(2+)](i)) with ion-sensitive microelectrodes. Unlike fluorescent indicators, these do not increase buffering. Slow injections of HCl (changing pH(i) by 0.1-0.2 pH ...
متن کاملEffects of Internal Divalent Cations on Voltage-Clamped Squid Axons
We have studied the effects of internally applied divalent cations on the ionic currents of voltage-clamped squid giant axons. Internal concentrations of calcium up to 10 mM have little, if any, effect on the time-course, voltage dependence, or magnitude of the ionic currents. This is inconsistent with the notion that an increase in the internal calcium concentration produced by an inward calci...
متن کاملCalcium entry induced by acetylcholine action on snail neurons.
A study was made of excitatory and inhibitory responses elicited by acetylcholine (ACh) in neurons of the snail Eobania vermiculata. At resting potential, ACh evoked a depolarizing inward current in some neurons (D-cells) and a hyperpolarizing current in others (H-cells). The currents elicited by ACh were nonlinearly dependent on membrane potential. After either D- or H-cells were equilibrated ...
متن کاملThe modulatory effects of orexin B on the calcium channels activity in neuronal cells of Helix aspersa (garden snail)
Introduction: The functional effects of orexin-B on the calcium spikes and excitability of the neuronal soma membrane of garden snail, Helix aspersa were studied. Methods: Conventional intracellular recording, under the current clamp conditions was performed to examine the effects of orexin-B on the configuration and electrophysiological properties of calcium spikes. Results: Application o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1996
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(96)79778-3