Efficient 5′-End Labeling of Oligonucleotides Containing Self-Complementary Sequences
نویسندگان
چکیده
منابع مشابه
Efficient 5'-end labeling of oligonucleotides containing self-complementary sequences.
Synthetic oligonucleotides have become basic tools in molecular biology research and are commonly used for cloning and characterization of genes, analysis of gene expression and so on. Frequently, the success of techniques such as DNA and RNA sequencing, screening of libraries, nuclease S1 and RNase protection assays or polymerase chain reaction (PCR) amplification depends on appropriate design...
متن کاملA general and efficient approach for the construction of RNA oligonucleotides containing a 5′-phosphorothiolate linkage
Oligoribonucleotides containing a 5'-phosphorothiolate linkage have provided effective tools to study the mechanisms of RNA catalysis, allowing resolution of kinetic ambiguity associated with mechanistic dissection and providing a strategy to establish linkage between catalysis and specific functional groups. However, challenges associated with their synthesis have limited wider application of ...
متن کاملSynthesis of DNA oligonucleotides containing 5-(mercaptomethyl)-2'-deoxyuridine moieties.
Recently thiolated oligonucleotides have attracted significant interest due to their ability to efficiently undergo stable bond formation with gold nanoparticles and surfaces to form DNA conjugates. In this respect we became interested in the synthesis of oligonucleotides that bear short thioalkyl functions located at the nucleobase. Here we present a strategy for the synthesis of DNA oligonucl...
متن کاملMixed backbone antisense oligonucleotides: design, biochemical and biological properties of oligonucleotides containing 2'-5'-ribo- and 3'-5'-deoxyribonucleotide segments.
We have designed and synthesized mixed backbone oligonucleotides (MBOs) containing 2'-5'-ribo- and 3'-5'-deoxyribonucleotide segments. Thermal melting studies of the phosphodiester MBOs (three 2'-5'linkages at each end) with the complementary 3'-5'-DNA and -RNA target strands suggest that 2'-5'-ribonucleoside incorporation into 3'-5'-oligodeoxyribonucleotides reduces binding to the target stran...
متن کاملSpecific and reversible photochemical labeling of plasmid DNA using photoresponsive oligonucleotides containing 3-cyanovinylcarbazole.
To develop a covalent and specific labeling method for single- and double-stranded plasmid DNA, photoresponsive oligonucleotide containing 3-cyanovinylcarbazole nucleoside was adopted. Single- and double-stranded plasmid DNA was successfully labeled/de-labeled with Cy3 and/or biotin by photoirradiation.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 1997
ISSN: 0736-6205,1940-9818
DOI: 10.2144/97234bm25