Efficient, Vpx independent shRNA transduction of monocyte derived dendritic cells as a tool for studying HIV transmission to primary T-cells
نویسندگان
چکیده
منابع مشابه
Vpx-Independent Lentiviral Transduction and shRNA-Mediated Protein Knock-Down in Monocyte-Derived Dendritic Cells
The function of dendritic cells (DCs) in the immune system is based on their ability to sense and present foreign antigens. Powerful tools to research DC function and to apply in cell-based immunotherapy are either silencing or overexpression of genes achieved by lentiviral transduction. To date, efficient lentiviral transduction of DCs or their monocyte derived counterparts (MDDCs) required hi...
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Human peripheral blood monocytes (HPBM) were cultured in the absence of human serum and were converted into a state exhibiting a high accessory function expressed by their ability of supporting lymphocyte proliferation. After a prolonged culture in serum free media the monocyte derived cells were highly viable, increased in size and developed veils and dendritiform elongatio'l1s. Paralleli...
متن کاملMonocyte-derived dendritic cells
The elicitation of efficient antitumor immune responses requires the optimal activation of tumor-associated dendritic cells (DCs). Our comparison of the effect of various immunostimulatory treatments on DCs revealed that the best predictor of the success of immunotherapy is not the activation of existing DC populations, but the appearance of a population of monocyte-derived DC in tumor-draining...
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In this paper, the mathematical model of HIV infection for CD8+ T-cells is illustrated. The homotopy analysis method and the Laplace transformations are combined for solving this model. Also, the convergence theorem is proved to demonstrate the abilities of presented method for solving non-linear mathematical models. The numerical results for $N=5, 10$ are presented. Several $hbar$-c...
متن کاملdifferentiation of monocyte derived dendritic cells in serum free conditions
human peripheral blood monocytes (hpbm) were cultured in the absence of human serum and were converted into a state exhibiting a high accessory function expressed by their ability of supporting lymphocyte proliferation. after a prolonged culture in serum free media the monocyte derived cells were highly viable, increased in size and developed veils and dendritiform elongatio'l1s. paralleli...
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ژورنال
عنوان ژورنال: Retrovirology
سال: 2013
ISSN: 1742-4690
DOI: 10.1186/1742-4690-10-s1-p100