EGFR Membrane Dynamics and Organization Investigated by Camera-Based Multi-Parameter Fluorescence Imaging with High Spatiotemporal Resolution
نویسندگان
چکیده
منابع مشابه
Multicolor Super-Resolution Fluorescence Imaging via Multi-Parameter Fluorophore Detection
Understanding the complexity of the cellular environment will benefit from the ability to unambiguously resolve multiple cellular components, simultaneously and with nanometer-scale spatial resolution. Multicolor super-resolution fluorescence microscopy techniques have been developed to achieve this goal, yet challenges remain in terms of the number of targets that can be simultaneously imaged ...
متن کاملProbing subtle fluorescence dynamics in cellular proteins by streak camera based Fluorescence Lifetime Imaging Microscopy
We report the cell biological applications of a recently developed multiphoton fluorescence lifetime imaging microscopy system using a streak camera (StreakFLIM). The system was calibrated with standard fluorophore specimens and was shown to have high accuracy and reproducibility. We demonstrate the applicability of this instrument in living cells for measuring the effects of protein targeting ...
متن کاملPixel multiplexing for high-speed multi-resolution fluorescence imaging
We introduce a imaging modality that works by transiently masking image-subregions during a single exposure of a CCD frame. By offsetting subregion exposure time, temporal information is embedded within each stored frame, allowing simultaneous acquisition of a full high spatial resolution image and a high-speed image sequence without increasing bandwidth. The technique is demonstrated by imagin...
متن کاملMulti-camera Imaging System Using Super-Resolution
This work presents a high-resolution image acquisition system by combining a multi-camera imaging system composed of multiple low-resolution digital cameras and a color super-resolution image reconstruction algorithm. Aligned and adjusted multiple mini-cameras and the controlling system consist the multi-camera imaging system. Some of the cameras obtain intensity information and the other ones ...
متن کاملUltra-high resolution imaging by fluorescence photoactivation localization microscopy.
Biological structures span many orders of magnitude in size, but far-field visible light microscopy suffers from limited resolution. A new method for fluorescence imaging has been developed that can obtain spatial distributions of large numbers of fluorescent molecules on length scales shorter than the classical diffraction limit. Fluorescence photoactivation localization microscopy (FPALM) ana...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2020
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2019.11.2583