Electron Microscope Study of DNA-Containing Plasms
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چکیده
منابع مشابه
Electron Microscope Study of DNA-Containing Plasms
The nucleoids of Escherichia coli, independently of the physiological state of the bacteria, are shown to be preserved as a fine-stranded fibrillar nucleoplasm by an OsO(4) fixation under defined conditions: acetate-veronal buffer pH 6, presence of Ca(++) and amino acids, stabilization with uranyl-acetate before dehydration. The same fixation procedure applied to the DNA of vegetative phage rev...
متن کاملElectron Microscope Study of DNA-Containing Plasms II. Vegetative and Mature Phage DNA as Compared with Normal Bacterial Nucleoids in Different Physiological States*
The nucleoids of Eseherichia coil, independently of the physiological state of the bacteria, are shown to be preserved as a fine-stranded fibrillar nucleoplasm by an OsO4 fixation under defined conditions: acetate-veronal buffer pH 6, presence of Ca ++ and amino acids, stabilization with uranyl-acetate before dehydration. The same fixation procedure applied to the DNA of vegetative phage reveal...
متن کاملKellenberger E, Ryter A & Séchaud J. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J. Biophys. Biochem. Cytol. 4:671-8, 1958
Under specific conditions—later called the 'R.K.-conditions'—bacterial nucleoids can be fixed with OsO4 so as to contain fine fibrillar material when viewed on thin sections. Under other conditions, and —as was shown later —with aldehydes, aggregations occur. Those induced with EDTA (formerly under the trade name 'versene'} allow a practical aggregation test. The pool of vegetative ( = replicat...
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HE limitation in the penetrating power of electrons is one of the most T serious obstacles in the study of chromosomes by electron microscopy. Chromosomes are generally larger than 0.2p in diameter and are nearly opaque to electron penetration. At various stages of the nuclear cycle, however, chromosomes change their size and shape. Dividing chromosomes are generally largest and “resting” chrom...
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ژورنال
عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology
سال: 1958
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.4.6.671