Embryo culture - Methods and protocols

Embryo Culture Challenge: Microbial Contamination

The success rate of embryo production critically depends on several factors. One of the pivotal factors is a technique used for the whole procedure of in vitro fertilization (IVF) and in vitro embryo culture (IVC) (1, 2). Besides the technique selected for IVF / in vitro embryo production (IVEP), there is an important consideration called IVF/IVEP culture dishes disin...

Bacterial diversity determination using culture-dependent and culture-independent methods

Mud volcanoes are taken into consideration by geologists and oil industry experts have given their association with oil and gas reserves and methane greenhouse gas production in hydrosphere and atmosphere. Gomishan mud volcano phenomenon in the southeastern edge of the Caspian Sea, given its oil and gas resources, has been studied by some geologists in terms of geology and tectonics but not in ...

Extending the limits of avian embryo culture with the modified Cornish pasty and whole-embryo transplantation methods.

The New and Early Chick (EC) methods, two commonly used techniques for ex ovo culture of early-stage avian embryos, are limited by poor survivability after initiation of circulation. This limitation is circumvented with two recent technical advancements: the modified Cornish pasty culture and whole-embryo transplantation. The former supports optimal ex ovo growth till stage HH18, and the latter...

In Vivo Embryo Culture Device

Z.P. Nagy et al. (eds.), Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices, DOI 10.1007/978-1-4419-1780-5_19, © Springer Science+Business Media, LLC 2012 Abstract The INVO procedure using the INVOcell device is a simple technique of human assisted reproduction. The fertilization of the oocytes and the early development of the embryos occur in the INVOcell device pla...

Embryo culture, assessment, selection and transfer