EMBRYONIC MOSAICISM IN PREIMPLANTATION GENETIC TESTING (PGT): EVALUATION OF VARIABLES THAT COULD AFFECT ITS FREQUENCY
نویسندگان
چکیده
Next-generation sequencing (NGS) for PGT has improved the resolution detecting mosaicism, which creates challenging embryo transfer decisions. Currently, there is a growing bulk of reports about mosaic transfers and their clinical outcomes. We consider that evidence still limited regarding factors could be related to risk mosaicism outcome after transfer. This study sought understand if embryos frequency are different variables report single-center retrospective included 2174 blastocysts tested between 2016 2020 by NGS (Veriseq, Illumina). DNA obtained from trophectoderm biopsies was in our laboratory were classified euploid, aneuploid, or mosaic. The divided into groups based on levels: low grade (20-40%), high (41-80%), chromosomal constitution (single, double, complex aneuploidy). analyzed according female age (<30, 30-34, 35-39, >40 years), indication (advanced maternal age, recurrent miscarriages, repeated IVF failure, patient request, translocations, others), day biopsy (5, 6 7), male factor, quality: (3-6AA 4-6AB, 3-6BA), mid (any BB, 1-3AB, 1-2AA 1-2BA) AC, CA, BC, CB CC). A Chi-square test applied statistical analysis. distributions studied determined. In addition, reproductive outcomes transferred date positive serum βhCG, ongoing pregnancy, live births. 891 (41%) out 2,174 702 (32%) 521 (24%) mosaic, 60 cases (3%) amplification failed. When embryonic variables, significant differences (p <0.05) found oocyte study. we grade, complex, observe they distributed across all mosaics most frequent ones each variable. Regarding outcomes, 50% 22 embryos, 23% had an pregnancy this resulted birth. On other hand, considering 391 euploid center, 56% 42% 33% Our results suggested associated with lower frequent. However, identification mainly low-grade ones, due methodological artifacts, therefore prospective including more samples will validate these results.
منابع مشابه
Preimplantation Genetic Testing
PLEASE NOTE: Contracts exclude from coverage, among other things, services or procedures that are considered investigational or cosmetic. Providers may bill members for services or procedures that are considered investigational or cosmetic. Providers are encouraged to inform members before rendering such services that the members are likely to be financially responsible for the cost of these se...
متن کاملPreimplantation Genetic Testing
Preimplantation genetic testing (PGT) involves analysis of biopsied cells as part of an assisted reproductive procedure. It is generally considered to be divided into 2 categories. Preimplantation genetic diagnosis (PGD) is used to detect a specific inherited disorder and aims to prevent the birth of affected children in couples at high risk of transmitting a disorder. Preimplantation genetic s...
متن کاملPreimplantation Genetic Testing
PLEASE NOTE: Contracts exclude from coverage, among other things, services or procedures that are considered investigational or cosmetic. Providers may bill members for services or procedures that are considered investigational or cosmetic. Providers are encouraged to inform members before rendering such services that the members are likely to be financially responsible for the cost of these se...
متن کاملO-27: Genome Instabilities in Preimplantation Development Leading to Genetic Variation between Tissues of Normal Human Fetuses
Background: Origin of midlife copy number variations (CNVs) between tissues in non-genetic diseases is unknown. Such genomic differences caused by post-zygotic events. They might either happen during the life or due to prevalent mosaicism in preimplantation stage. We aim to explore fetal mosaicism and its origins. Materials and Methods: Two apparently normal fetuses were achieved following the ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Fertility and Sterility
سال: 2021
ISSN: ['0015-0282', '1556-5653']
DOI: https://doi.org/10.1016/j.fertnstert.2021.07.1039