Energy-dependent degradation of lambda O protein in Escherichia coli

Advances and challenges in recombinant protein expression in Escherichia coli

Effects of reduced energy production on protein degradation, guanosine tetraphosphate, and RNA synthesis in Escherichia coli.

In Escherichia coli (strain A33, ATCC No. 27873) protein degradation increases and RNA synthesis falls under conditions that reduce the cell’s capacity to generate ATP (e.g. glucose or phosphate starvation). To test whether reduced energy production might signal these responses, cellular ATP content was lowered to different extents with various concentrations of inhibitors of electron transport...

FtsH-dependent degradation of phage shock protein C in Yersinia enterocolitica and Escherichia coli.

The widely conserved phage shock protein (Psp) extracytoplasmic stress response has been studied extensively in Escherichia coli and Yersinia enterocolitica. Both species have the PspF, -A, -B, and -C proteins, which have been linked to robust phenotypes, including Y. enterocolitica virulence. PspB and PspC are cytoplasmic membrane proteins required for stress-dependent induction of psp gene ex...

Crosstalk between Diverse Synthetic Protein Degradation Tags in Escherichia coli.

Recently, a synthetic circuit in E. coli demonstrated that two proteins engineered with LAA tags targeted to the native protease ClpXP are susceptible to crosstalk due to competition for degradation between proteins. To understand proteolytic crosstalk beyond the single protease regime, we investigated in E. coli a set of synthetic circuits designed to probe the dynamics of existing and novel d...

N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli

Dynamically altering protein concentration is a central activity in synthetic biology. While many tools are available to modulate protein concentration by altering protein synthesis rate, methods for decreasing protein concentration by inactivation or degradation rate are just being realized. Altering protein synthesis rates can quickly increase the concentration of a protein but not decrease, ...