Energy-linked Changes of Hydrogen Ion Concentration in Submitochondrial Particles
نویسندگان
چکیده
منابع مشابه
Energy-linked Ion Tramlocation in Submitochondrial Particles
1. Ca++ accumulation was shown to take place at pH 7.0 in submitochondrial particles. The particles were prepared from bovine heart mitochondria either by digitonin treatment or by sonic oscillation followed by osmotic shock and sedimentation at 26,000 X g. Both succinate and ATP (or ADP) were required for Ca++ accumulation. Carbonylcyanide ptrifluoromethoxyphenylhydrazone abolished the process...
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1. Bromthymol blue added to rat liver mitochondria at concentrations in the range 0.4 to 4.0 mpmoles per mg of protein is tightly bound as indicated by centrifugation and washing of the mitochondria. 2. The absorbance of the dye is increased on adding Ca++ or Mn++ to the mitochondria in the absence of a permeant anion. The extent of increase is 0.03 cm-l for 167 PM Ca++ at 618 rnp for 0.5 mpmol...
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A respiratory chain inhibitor antimycin has long been known to bring about a shift of the ferrous cytochrome b-562 a-absorption band by about l-2 nm to the red [l-3]. This red shift was sometimes considered to be a specific effect of antimycin [4,5] since it had not been observed in animal mitochondria with an antimycin-type inhibitor HOQNO [3]. At the same time, rather similar perturbations of...
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Oxygen exchange reactions in sonically prepared submitochondrial particles show a nearly absolute dependence upon added adenine nucleotides. The orthophosphate = adenosine triphosphate and the ATP e HOH exchange reactions, in contrast to the Pi s HOH exchange, are favored by presence of ADP rather than ATP, are more sensitive to p-mercuribenzoate and to 2,4-dinitrophenol, and require a higher M...
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Submitochondrial particles from mung bean mitochondria (Phaseolus aureus) are able to catalyze an energy-linked reduced nicotinamide adenine dinucleotide-nicotinamide adenine dinucleotide phosphate transhydrogenase reaction supported by ATP or by aerobically generated high energy intermediates. The energy transfer pathway appears to differ from that utilized for oxidative phosphorylation.Mung b...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96200-9