Enhancement of 2,3-butanediol production by Klebsiella pneumoniae PTCC 1290: Application of Taguchi methodology for process optimization
نویسندگان
چکیده
منابع مشابه
Extractive Capacity of Oleyl Alcohol on 2, 3-Butanediol Production in Fermentation Process with Use of Klebsiella pneumoniae PTCC 1290
Recovery of metabolites from fermentation broth by solvent extraction can be used to optimize fermentation processes. End-product reutilization, low product concentration and large volumes of fermentation broth and the requirements for large bioreactors, in addition to the high cost largely contributed to the decline in fermentative 2,3-butanediol production. Extraction can successfully be ...
متن کاملEnhancement of 2,3-Butanediol Production by Klebsiella oxytoca PTCC 1402
Optimal operating parameters of 2,3-Butanediol production using Klebsiella oxytoca under submerged culture conditions are determined by using Taguchi method. The effect of different factors including medium composition, pH, temperature, mixing intensity, and inoculum size on 2,3-butanediol production was analyzed using the Taguchi method in three levels. Based on these analyses the optimum conc...
متن کاملextractive capacity of oleyl alcohol on 2, 3-butanediol production in fermentation process with use of klebsiella pneumoniae ptcc 1290
recovery of metabolites from fermentation broth by solvent extraction can be used to optimize fermentation processes. end-product reutilization, low product concentration and large volumes of fermentation broth and the requirements for large bioreactors, in addition to the high cost largely contributed to the decline in fermentative 2,3-butanediol production. extraction can successfully be used...
متن کاملRedistribution of Carbon Flux toward 2,3-Butanediol Production in Klebsiella pneumoniae by Metabolic Engineering
Klebsiella pneumoniae KCTC2242 has high potential in the production of a high-value chemical, 2,3-butanediol (2,3-BDO). However, accumulation of metabolites such as lactate during cell growth prevent large-scale production of 2,3-BDO. Consequently, we engineered K. pneumoniae to redistribute its carbon flux toward 2,3-BDO production. The ldhA gene deletion and gene overexpression (budA and budB...
متن کاملProcess Optimization on Micro-Aeration Supply for High Production Yield of 2,3-Butanediol from Maltodextrin by Metabolically-Engineered Klebsiella oxytoca
An optimization process with a cheap and abundant substrate is considered one of the factors affecting the price of the production of economical 2,3-Butanediol (2,3-BD). A combination of the conventional method and response surface methodology (RSM) was applied in this study. The optimized levels of pH, aeration rate, agitation speed, and substrate concentration (maltodextrin) were investigated...
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ژورنال
عنوان ژورنال: African Journal of Biotechnology
سال: 2009
ISSN: 1684-5315
DOI: 10.5897/ajb2009.000-9477