Enhancing the detection of Toxoplasma gondii via an anti-SAG1 scFv-alkaline phosphatase immunoconjugate
نویسندگان
چکیده
منابع مشابه
Recombinant scFv Antibodies against P30 Surface Protein of Toxoplasma Gondii
Background & Aims: Toxoplasma gondii is an obligate, intracellular parasite, which is widely spread in the world. The parasite is able to infect all warm-blooded hosts including humans and farm animals. The infection in humans often occurs after the ingestion of raw or undercooked meat containing tissue cysts. Several methods have been applied to detect this parasite in contaminated foods. Reco...
متن کاملDetection of genomic Toxoplasma gondii DNA and anti-Toxoplasma antibodies
Toxoplasmosis is a disease of zoonotic nature; being reported to be widespread in animals and humans. Serological diagnosis represents the first and the most widely used approach to define the stage of toxoplasmosis and diagnosis of primary and late infection in pregnancy can be improved by determination of Toxoplasma DNA. Eighty-eight and 88 coagulated and non coagulated blood samples were col...
متن کاملEvaluation of Recombinant SAG1 Protein for Detection of Toxoplasma gondii Specific Immunoglobulin M by ELISA Test
BACKGROUND Toxoplasmosis is a serious disease in immunocompromised patients and pregnant women. Differentiation of acute and chronic infection is a major challenge in serodiagnosis of the disease. Since the aim of this study was to assess the diagnostic utility of recombinant SAG1 (rec-SAG1) for the detection of Toxoplasma-specific IgM antibodies in human sera, by an enzyme-linked immunosorbent...
متن کاملPrimary structure of mature SAG1 gene of an Indonesian Toxoplasma gondii and comparison with other strains
Toxoplasma gondii is a persistent protozoan parasite capable of infecting almost any warm-blooded vertebrates. SAG1 (p30) is the prototypic member of a superfamily of surface antigens called SRS (SAG1-related sequence). It constitutes the most abundant and predominant antigen. In this paper the primary structure of mature SAG1 gene of an Indonesian T. gondii isolate is described and sequence co...
متن کاملSerologic Detection of Anti Toxoplasma gondii Infection in Diabetic Patients
BACKGROUND Toxoplasmosis is caused by the Toxoplasma gondii parasite. The parasite is intracellular and can result in severe complications leading to death in immuno-deficient patients in particular. Diabetes is an important factor that increases susceptibility and risk of various infections in the host. OBJECTIVES The present study focused on the serologic detection of Toxoplasma gondii infe...
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ژورنال
عنوان ژورنال: Biotechnology Reports
سال: 2019
ISSN: 2215-017X
DOI: 10.1016/j.btre.2019.e00360