Enzymatic Hydrolysis Strategies for Cellulosic Sugars Production to Obtain Bioethanol from Eucalyptus globulus Bark

Cellulosic sugars production for the valorization of lignocellulosic biomass residues in an industrial site has economic benefits and is promising if integrated into a biorefinery. Enzymatic hydrolysis (EH) pretreated Eucalyptus globulus bark, residue low-economic value widely available Portuguese pulp paper mills, could be excellent approach to attain resource circularity mill profitability. This work evaluated potential improving cellulosic concentrations by operating with high solids loading introducing additives Triton X-100, PEG 4000 Tween 80 using commercial enzymatic consortium dosage 25 FPU gcarbohydrates−1. Additives did not improve performance, but effect increasing 14% (w/v) batch operation was accomplished. The fed-batch strategy investigated and, when starting 11% loading, allowed feeding 3% fresh feedstock sequentially at 2, 4 6 h, attaining 20% total loading. After 24 h operation, concentration reached 161 g L−1, corresponding EH conversion efficiency 76%. Finally, fermentability hydrolysate Ethanol Red® strain 5 L bioreactor scale. present results demonstrate that previously kraft pulping, production, allowing it become raw material wide range bioprocesses.