Enzymatic microdetermination method for galactocerebrosidase (EC 3.2.1.46) in tissue samples.

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Rapid method for homogenizing tissue samples for liquid scintillation counting.

EVALUATiON OF A new drug includes analysis of its tissue distribution. We have developed an improved method for solubilizing tissues that is not only rapid, but results in a homogeneous sample for counting. We had found that organs rich in connective tissue (heart, lung, skeletal muscle, urinary bladder, stomach, and intestinal wall) could not be completely disrupted by conventional techniques ...

متن کامل

Resorcinol Method for Colorimetric Microdetermination of Copper in Pure Forms

A simple colorimetric method is proposed for the micro-determination of copper in pure forms. The assay is based on the formation of a color complex between copper and resorcinol in presence of ammonia with peak absorption at 450 nm. The method is linear over 0.05 through 1.0 μg Cu (R ≥0.99). The regression coefficients vary from 0.275 to 0.920 depending upon the choice of experimental conditio...

متن کامل

[A modified method for the microdetermination of citric acid].

An investigation of the different steps in the method of Weil-Malherbe & Bone (1949), as modified by Taylor (1953), has led to an altered procedure for citric acid determination, with increased sensitivity and accuracy. The main modifications involved are the use of (i) a higher concentration of sulphuric acid during the formation of pentabromoacetone, and (ii) a thiourea-borax-sodium sulphide ...

متن کامل

Enzymatic fluorometric methods for the microdetermination of hexose phosphates in muscle.

Our current investigations on the chemical processes associated with muscular activity (1) require more sensitive methods for the determination of various metabolic intermediates than are currently available, and the present work is a contribution to the development of such procedures. These aim at the determination of hexose monophosphates on the one hand, fructose diphosphate and triose phosp...

متن کامل

An enzymatic cycling method for the measurement of myo-inositol in biological samples.

INTRODUCTION A sensitive and simple enzymatic cycling method is described for the quantitation of myo-inositol in biological samples. METHODS The method involves the use of a sensitive and simple enzymatic cycling method is described for the quantitation of myo-inositol in biological samples. The method involves use of thio-NAD(+), NADH and thermostable myo-inositol dehydrogenase (IDH; EC. 1....

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Proceedings of the Japan Academy, Series B

سال: 1979

ISSN: 0386-2208,1349-2896

DOI: 10.2183/pjab.55.69