Enzymatic Mutation Detection in the P53 Gene
نویسندگان
چکیده
منابع مشابه
Enzymatic mutation detection in the P53 gene.
BACKGROUND The enzymatic mutation detection (EMD) assay uses the bacteriophage resolvase T4 endonuclease VII, which cleaves preformed heteroduplex molecules at mismatch sites, forming two shorter fragments that can be resolved by gel electrophoresis. The method can be used to detect single and multiple base changes, as well as insertions and deletions. METHODS The sensitivity, specificity, an...
متن کاملEnzymatic mutation detection method evaluated for detection of p53 mutations in cDNA from breast cancers.
BACKGROUND Rapid, reproducible, and easily run methods with high sensitivity and specificity are required for mutation screening of clinical samples. We evaluated the Enzymatic Mutation Detection (EMD(TM)) method by analysis of archival cDNA from 203 breast cancer patients and comparison with results of cDNA-based sequencing of the tumor suppressor gene p53. METHODS The EMD technology uses th...
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Objective Mustard gas (MG) is a poisoning chemical, mutagenic and carcinogenic alkylating agent. It is used during World War I and also Iran-Iraq conflict. The p53 tumor suppressor gene is involved in the pathogenesis of malignant disease. The aim of this study is to determine possible mutation in p53 gene of lung sample from mustard gas exposed patients. Material and Methods Twelve lung bio...
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In order to clarify the significance of mutation of the p53 tumor suppressor gene in the genesis and development of human hepatocellular carcinoma (HCC) in an aflatoxin I!, Ion-exposure area, the spec trum, i.e.. incidence, type, and site, oipS3 gene mutations was examined in 169 tissue samples resected mainly from Japanese patients using single-strand conformation polymorphism analysis and dir...
متن کاملdetection of somatic mutation of codon 248 of p53 gene between iranian women with breast cancer
abstract objective: detection of p53 gene mutation on its codon 248 between iranian women with breast cancer by pcr-rflp. material and methods: dna extraction of tumor tissues was performed in 53 patients with primary breast cancer. designing primer for the sub-studying codon was performed with nti-vector software and detection of codon 248 mutation was done by msp i pcr_rflp. results: the soma...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2000
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/46.10.1562