Enzymatic synthesis of D-cysteine by 3-chloro-D-alanine resistant pseudomonas putida CR 1-1.
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چکیده
منابع مشابه
Inactivation of Bacterial D-Amino Acid Transaminase by fi-Chloro-D-alanine*
Purified D-amino acid transaminase from Bacillus sphaericus catalyzes an (~,/3 elimination from the D isomer of pchloroalanine to yield equivalent amounts of pyruvate, chloride, and ammonia; the L isomer of chloroalanine is not a substrate for this transaminase. During the /I elimination there is a synchronous loss in enzyme activity; the Kinact for /I-chloroalanine was estimated to be about 10...
متن کاملD 2 . 1 . 3 . 1
With contributions from: Maarten Menken, Heiner Stuckenschmidt, Holger Wache (Vrije Universiteit Amsterdam) Luciano Serafini (Centro per la Ricerca Scientifica e Tecnologica, Trento) Andrei Tamilin (Università degli Studi di Trento) Mustafa Jarrar (Vrije Universiteit Brussel) Fabio Porto (Ecole Polytechnique Fédérale de Lausanne) Christine Parent (Université de Lausanne) Alan Rector, Jeff Pan (...
متن کاملInactivation of bacterial D-amino acid transaminase by beta-chloro-D-alanine.
Purified D-amino acid transaminase from Bacillus sphaericus catalyzes an alpha,beta elimination from the D isomer of beta-chloroalanine to yield equivalent amounts of pyruvate, chloride, and ammonia; the L isomer of chloroalanine is not a substrate for this transaminase. During the beta elimination there is a synchronous loss in enzyme activity; the Kinact for beta-chloroalanine was estimated t...
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Two biologically active insulin analogs, [l+AlanineA] ([L-Ala’-A]) and [1-o-Alanine-A] ([o-Ala’-A]), which differ from the parent molecule in that the NHz-terminus of the A chain (A’), glycine, has been replaced by Land o-alanine, respectively, were synthesized. For this purpose, the [L-Ala’]A and [n-Ala’]A chains were synthesized by the fragment condensation approach and isolated in the S-sulf...
متن کاملAffinity labeling of alanine aminotransferase by 3-chloro-L-alanine.
The pyridoxal form of alanine aminotransferase from pig heart catalyzes the a,/? elimination reaction with 3chloro-L-alanine as the substrate to form equimolar amounts of pyruvate, ammonia, and chloride. The maximum rate of the a,/3 elimination reaction was 2.5 pmol/min/mg at pH 7.0 (25”(Z), approximately 0.5% that of the transamination reaction between L-alanine and 2-oxoglutarate. Time-depend...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1982
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.46.3003