Enzymes as biosensors. 2. Hysteretic response of chloroplastic fructose-1, 6-bisphosphatase to fructose 2, 6-bisphosphate
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چکیده
منابع مشابه
Fructose-2,6-Bisphosphate synthesis by 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase 4 (PFKFB4) is required for the glycolytic response to hypoxia and tumor growth
Fructose-2,6-bisphosphate (F2,6BP) is a shunt product of glycolysis that allosterically activates 6-phosphofructo-1-kinase (PFK-1) resulting in increased glucose uptake and glycolytic flux to lactate. The F2,6BP concentration is dictated by four bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (PFKFB1-4) with distinct kinase:phosphatase activities. PFKFB4 is over-expressed in ...
متن کاملExpression of the liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase mRNA in FAO-1 cells.
The hormonal regulation of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene expression was studied in the rat hepatoma cell line FAO-1. Both 6-phosphofructo-2-kinase and fructose-2,6-bisphosphatase activities were detected in FAO-1 cells, at 68% of the levels found in rat liver. Northern blot analysis showed that FAO-1 cells, like rat liver, contained a predominant species of bifunctio...
متن کاملFructose - l , 6 - Bisphosphate 1 s an Allosteric Activator of Pyrophosphate : Fructose - 6 - Phosphate 1 - Phosphotransferase ' Tom
The activity of highly purified pyrophosphate:fructose-6-phosphate 1 -phosphotransferase (PFP) from barley (Hordeum vulgare) leaves was studied under conditions where the catalyzed reaction was allowed to approach equilibrium. The activity of PFP was monitored by determining the changes in the levels of fructose-6phosphate, orthophosphate, and fructose-1,6-bisphosphate (Fru1,6-bisP). Under thes...
متن کاملArg-257 and Arg-307 of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase bind the C-2 phospho group of fructose-2,6-bisphosphate in the fructose-2,6-bisphosphatase domain.
Rat liver fructose-2,6-bisphosphatase, which catalyzes its reaction via a phosphoenzyme intermediate, is evolutionarily related to the phosphoglycerate mutase enzyme family (Bazan, F., Fletterick, R., and Pilkis, S.J. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 9642-9646). Arg-7 and Arg-59 of the yeast phosphoglycerate mutase have been postulated to be substrate-binding residues based on the x-ray...
متن کاملStudies on the Entry of Fructose - 2 , 6 - Bisphosphate into Chloroplasts 1
The regulatory metabolite fructose-2,6-bisphosphate (Fru-2,6P2) has an important function in controlling the intermediary carbon metabolism of leaves. Fru-2,6-P2 controls two cytosolic enzymes involved in the interconversion of fructose-6-phosphate and fructose-1,6-bisphosphate (fructose-1,6-bisphosphatase and pyrophosphate,fructose-6-phosphate 1-phosphotransferase) and thereby controls the par...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1988
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1988.tb14257.x