Escherichia coli strains with nonimmune immunoglobulin-binding activity

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Escherichia coli strains with nonimmune immunoglobulin-binding activity.

We have identified several strains of Escherichia coli which contain immunoglobulin-binding activity on the cell surface. Affinity-purified antibodies ordinarily used as secondary antibodies in immunodetection protocols were bound by 6 of 72 strains of the ECOR reference collection of E. coli. The Fc fragments of both human and sheep immunoglobulin G (IgG) were also bound, demonstrating the non...

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Four different genes responsible for nonimmune immunoglobulin-binding activities within a single strain of Escherichia coli.

Certain Escherichia coli strains bind the Fc fragment of immunoglobulin G (IgG) at the bacterial cell surface. Previous work established that this nonimmune Ig binding depends on several large proteins with apparent molecular masses that can exceed 200 kDa. For E. coli strain ECOR-9, four distinct genes (designated eibA, eibC, eibD, and eibE) are responsible for Ig binding. Two eib genes are li...

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To successfully perform molecular genetic techniques it is essential to have a full understanding of the properties of the various Escherichia coli host strains commonly used for the propagation and manipulation of recombinant DNA. E. coli is an enteric rod-shaped Gram-negative bacterium with a circular genome of 4.6 Mb (1). It was originally chosen as a model system because of its ability to g...

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ژورنال

عنوان ژورنال: Infection and Immunity

سال: 1997

ISSN: 0019-9567,1098-5522

DOI: 10.1128/iai.65.11.4572-4579.1997