Establishment of a transient expression system forDictyostelium discoideum
نویسندگان
چکیده
منابع مشابه
Establishment of a transient expression system for Dictyostelium discoideum.
We have established a rapid and sensitive transient expression system for Dictyostelium discoideum. We constructed a gene fusion containing the promoter from the Dictyostelium Actin 15 gene fused to the firefly luciferase gene. The enzymatic activity of this gene fusion, expressed at very high levels in stable transformants, was measured to determine optimum conditions for transient expression ...
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چکیده ندارد.
15 صفحه اولDictyostelium Discoideum: A Developmental System
cernible. The peroxidase technique also permits the specimen to be examined by electron microscopy. Perhaps a few too many chapters are devoted to individual applications of the technique, but it is an exciting development in diagnostic virology. Viral genomes can be present in cells without full expression leading to the production of viral antigens or virions which can be detected by immunolo...
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Genetic studies of Ochrobactrum anthropi are hindered by the lack of a suitable gene expression system. We constructed a set of vectors containing several promoters and a His tag fusion in the N terminus to facilitate protein detection and purification. The new vectors should significantly enhance the genetic manipulation and characterization of O. anthropi.
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Inducible expression systems are essential for the expression of toxic proteins and are very convenient for proteins that induce strong side effects such as retardation of growth or development. Currently available systems for use in Dictyostelium either do not have a very tight control over expression levels or use a combination of an integrating and an extrachromosomal vector. We designed a n...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1988
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/16.6.2613