Estimating the Persistence Length of a Worm-Like Chain Molecule from Force-Extension Measurements
نویسندگان
چکیده
منابع مشابه
Estimating the persistence length of a worm-like chain molecule from force-extension measurements.
We describe a simple computation of the worm-like chain model and obtain the corresponding force-versus-extension curve. We propose an improvement to the Marko and Siggia interpolation formula of Bustamante et al (Science 1994, 265:1599-1600) that is useful for fitting experimental data. We apply it to the experimental elasticity curve of single DNA molecules. Finally, we present a tool to stud...
متن کاملAlternative spring force law for bead-spring chain models of the worm-like chain
e have developed a new spring force law which can be used in bead-spring chain models of the orm-like chain. The bead-spring chain has no bending potentials between the springs, and so iffers from the current models only in the functional form of the force law. This new model can ccurately represent a chain that contains many persistence lengths even if each spring represents nly a few persiste...
متن کاملSingle-molecule measurements of the persistence length of double-stranded RNA.
Over the past few years, it has become increasingly apparent that double-stranded RNA (dsRNA) plays a far greater role in the life cycle of a cell than previously expected. Numerous proteins, including helicases, polymerases, and nucleases interact specifically with the double helix of dsRNA. To understand the detailed nature of these dsRNA-protein interactions, the (bio)chemical, electrostatic...
متن کاملPersistence Length of Short-Chain Branched Polyethylene
The effect of short-chain branching (SCB) on the persistence length lp of polyethylene (PE) was studied using small-angle neutron scattering (SANS). In thermodynamically good solvents, lp can be measured directly from the scattering vector qtr at the crossover from good solvent mass-fractal scaling to the rodlike persistent scaling, using the unified equation described in the text. The method w...
متن کاملPersistence length measurements from stochastic single-microtubule trajectories.
We present a simple method to determine the persistence length of short submicrometer microtubule ends from their stochastic trajectories on kinesin-coated surfaces. The tangent angle of a microtubule trajectory is similar to a random walk, which is solely determined by the stiffness of the leading tip and the velocity of the microtubule. We demonstrate that even a single-microtubule trajectory...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1999
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(99)77207-3