Ethidium bromide binding to core particle: comparison with native chromatin
نویسندگان
چکیده
منابع مشابه
Binding of ethidium bromide causes dissociation of the nucleosome core particle.
The binding of ethidium bromide to chicken erythrocyte core particles results in a step-wise dissociation of the structure that involves the initial release of one copy each of histones H2A and H2B. Quantitation of the dissociated DNA reveals that a critical amount of drug is required for the dissociation. Above the critical value, the dissociation is time dependent, reversible, and independent...
متن کاملA study of the interaction between ethidium bromide and rye chromatin: comparison with calf thymus chromatin.
We studied the interaction of ethidium bromide with rye and calf thymus chromatin. Both types of chromatin have the same dye accessibility, which is about 50% of that of DNA. From this result we conclude that the molecular structure of these two chromatins is similar. For rye, the extraction of H1 produces no change in the binding of ethidium bromide. The subsequent extraction of H2A and H2B pr...
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This study compares some physical properties of DNA in native chromatin and mono-, di-, trinucleosomes obtained after mild micrococcal nuclease digestion. Melting curves and derivatives are shown to be very similar from one sample to another although a shift from 79 to 82 degrees C is observed between the mainly monophasic peak of multimers and chromatin. Careful analysis of the positive band o...
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Ethidium bromide and polylysine interact with nucleosomal DNA and lead to change of biochemical properties and to morphological changes as to the distance between the two core particles of a nucleosome dimer. With increasing polylysine concentration, the buoyant density of nucleosomes decrease and the accessibility of the nucleosomal DNA to micrococcal nuclease is lowered. Electron microscopy o...
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Binding of ethidium bromide to Escherichia coli tRNAVal and an RNA minihelix based on the acceptor stem and T-arm of tRNAVal was investigated by 19F and 1H NMR spectroscopy of RNAs labeled with fluorine by incorporation of 5-fluorouracil. Ethidium bromide selectively intercalates into the acceptor stem of the tRNAVal. More than one ethidium bromide binding site is found in the acceptor stem, th...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1979
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/6.10.3231