Eukaryotic DNA segments capable of autonomous replication in yeast.
نویسندگان
چکیده
منابع مشابه
DNA replication in eukaryotic cells.
The maintenance of the eukaryotic genome requires precisely coordinated replication of the entire genome each time a cell divides. To achieve this coordination, eukaryotic cells use an ordered series of steps to form several key protein assemblies at origins of replication. Recent studies have identified many of the protein components of these complexes and the time during the cell cycle they a...
متن کاملDNA Replication in Yeast
The details of chromosome replication are better understood in the budding yeast, Saccharomyces cerevisiae, than in any other eukaryotic organism. cis-Acting replicator sequences required for chromosomal replication origin function were identified on the basis of their ability to promote the extrachromosomal maintenance of plasmids. These autonomously replicating sequence (ARS) elements have be...
متن کاملIdentification of a cis-element that determines autonomous DNA replication in eukaryotic cells.
A 36-bp human consensus sequence (CCTMDAWKSGBYTSMAAWTWBCMYTTRSCAAATTCC) is capable of supporting autonomous replication of a plasmid after transfection into eukaryotic cells. After transfection and in vitro DNA replication, replicated plasmid DNA containing a mixture of oligonucleotides of this consensus was found to reiterate the consensus. Initiation of DNA replication in vitro occurs within ...
متن کاملThe DNA replication fork in eukaryotic cells.
Replication of the two template strands at eukaryotic cell DNA replication forks is a highly coordinated process that ensures accurate and efficient genome duplication. Biochemical studies, principally of plasmid DNAs containing the Simian Virus 40 origin of DNA replication, and yeast genetic studies have uncovered the fundamental mechanisms of replication fork progression. At least two differe...
متن کاملMaking sense of eukaryotic DNA replication origins.
DNA replication is the process by which cells make one complete copy of their genetic information before cell division. In bacteria, readily identifiable DNA sequences constitute the start sites or origins of DNA replication. In eukaryotes, replication origins have been difficult to identify. In some systems, any DNA sequence can promote replication, but other systems require specific DNA seque...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1980
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.77.8.4559