Evaluation of process parameters in shake flasks for mammalian cell culture

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Evaluation of process parameters in shake flasks for mammalian cell culture

Introduction Shake flask cultivation is nowadays a routine technique during process development for mammalian cell lines. During shaken culture, changes in agitation velocity, shaking diameter or shake flask size affect the hydrodynamics in the shake flask. This might be reflected in the growth of the cultured cells. Process parameters such as power input, mixing time, fluid velocity etc. have ...

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ژورنال

عنوان ژورنال: BMC Proceedings

سال: 2013

ISSN: 1753-6561

DOI: 10.1186/1753-6561-7-s6-p17