Excitement Over Automated Patch Clamp: Action Potentials from Cardiac Myocytes
نویسندگان
چکیده
منابع مشابه
Automated planar patch-clamp.
Ion channels are integral membrane proteins that regulate the flow of ions across the plasma membrane and the membranes of intracellular organelles of both excitable and non-excitable cells. Ion channels are vital to a wide variety of biological processes and are prominent components of the nervous system and cardiovascular system, as well as controlling many metabolic functions. Furthermore, i...
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(1.1) Cm ∂V ∂t = 1 Ra ∂2V ∂x2 − Iion(V, t)− Istim(t), where Ra and Cm are the axial resistance and membrane capacitance, Iion represents the total ionic current, and Istim(t) is an applied stimulus current which instigates an action potential. The source term Iion is usually specified by some Hodgkin-Huxley type ionic model. In our simulations, we use the Luo-Rudy phase I (1991) model, one of t...
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Ion channels are essential in a wide range of cellular functions and their malfunction underlies many disease states making them important targets in drug discovery. The availability of standardized cell lines expressing ion channels of interest lead to the development of diverse automated patch clamp (APC) systems with high-throughput capabilities. These systems are now available for drug scre...
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The cardiac late Na (+) current is generated by a small fraction of voltage-dependent Na (+) channels that undergo a conformational change to a burst-gating mode, with repeated openings and closures during the action potential (AP) plateau. Its magnitude can be augmented by inactivation-defective mutations, myocardial ischemia, or prolonged exposure to chemical compounds leading to drug-induced...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2010
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2009.12.2798