Exogenous growth differentiation factor 9 in oocyte maturation media enhances subsequent embryo development and fetal viability in mice

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Exogenous growth differentiation factor 9 in oocyte maturation media enhances subsequent embryo development and fetal viability in mice.

BACKGROUND Successful oocyte in vitro maturation (IVM) would eliminate the need for hormonal stimulation used in assisted reproduction techniques. Unfortunately, oocytes matured in vitro have compromised developmental competence possibly due to disrupted oocyte-cumulus communication resulting from inappropriate levels of oocyte-secreted factors such as growth differentiation factor 9 (GDF9). He...

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melatonin effect during different maturation stages of oocyte and subsequent embryo development in mice

background: it is important to protect oocytes and embryos from oxidative stress in the culture medium. melatonin has been shown to be a direct free radical scavenger. objective: effect of melatonin during in vitro oocyte maturation, fertilization and embryo development of mouse oocytes was evaluated. materials and methods: oocytes from supper-ovulated mouse were divided to two groups: cumulus ...

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Combined Effect of Retinoic Acid and Basic Fibroblast Growth Factor on Maturation of Mouse Oocyte and Subsequent Fertilization and Development

Objective Many autocrine and paracrine elements that are produced within follicular niche have been the focus of much in vitro maturation research. The present study was carried out to compare retinoic acid and basic fibroblast growth factor (bFGF) efficacy on IVM of mouse oocytes, and their further dual consumption to reach an optimal protocol. MaterialsAndMethods GV oocytes obtained from two-...

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P-214: The Rate of In Vitro Oocyte Maturation,Fertilization and Embryo Development in Presence of Melatonin in Mice

Background: It is important to protect oocytes and embryos from oxidative stress in the culture medium. Melatonin has been shown to be a direct free radical scavenger. Therefore, effect of melatonin during in vitro oocyte maturation (IVM), fertilization and embryo development of mouse oocytes was evaluated. Materials and Methods: Oocytes from supper-ovulated mouse were divided to two groups: cu...

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Melatonin effect during different maturation stages of oocyte and subsequent embryo development in mice

BACKGROUND It is important to protect oocytes and embryos from oxidative stress in the culture medium. Melatonin has been shown to be a direct free radical scavenger. OBJECTIVE Effect of melatonin during in vitro oocyte maturation, fertilization and embryo development of mouse oocytes was evaluated. MATERIALS AND METHODS Oocytes from supper-ovulated mouse were divided to two groups: cumulus...

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ژورنال

عنوان ژورنال: Human Reproduction

سال: 2007

ISSN: 0268-1161,1460-2350

DOI: 10.1093/humrep/dem140