Experimental tools to reduce the burden of bacterial synthetic biology

Cellular burden limits the applications of bacterial synthetic biology. Experimental approaches for minimisation have recently become available. Tools to identify construct design with low footprint on host include capacity monitors that quantify cellular capacity, high-throughput and cell-free systems prototyping. Orthogonal ribosomes feedback controllers are instead useful seek control resource allocation lower burden. Other genome reduction increase available pool addiction couple cell fitness product accumulation. However, controlling response exogenous expression is still a challenge, more tools needed widen Further effort combines novel evolutionary data burden-aware can set foundation stability robustness future genetic systems. • strategies reduce in engineered cells. Capacity monitor, TX-TL DoE support part host. ribosome allocate resources minimise Genome engineering frees up boosting expression. Synthetic reduces phenotypic variability long-term bioprocesses. Robust challenge waiting further consideration.