Expression and Purification of Human Keratinocyte Growth Factor 2 by Fusion with SUMO
نویسندگان
چکیده
منابع مشابه
High-level expression and purification of human epidermal growth factor with SUMO fusion in Escherichia coli.
Human epidermal growth factor (hEGF) can stimulate the division of various cell types and has potential clinical applications. However, the high expression of active hEGF in Escherichia coli has not been successful, as the protein contains three intra-molecular disulfide bonds that are difficult to form correctly in the bacterial intracellular environment. To solve this problem, we fused the hE...
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results cloning was confirmed by pcr and restriction digestion. rt-pcr and sds-page represented expression of kgf in e. coli. the optimized expression was achieved 16 hours after induction with 0.3 mm iptg at 37°c in luria broth (lb) containing kanamycin. the 18 kda protein was confirmed by western blotting, using anti-his antibodies. conclusions the result of the present study indicated that e...
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ژورنال
عنوان ژورنال: Molecular Biotechnology
سال: 2008
ISSN: 1073-6085,1559-0305
DOI: 10.1007/s12033-008-9135-7