Expression and visualization of Green Fluorescent Protein (GFP) in Neurospora crassa

Endogenous green fluorescent protein (GFP) in amphioxus.

Discovery of Green Fluorescent Protein, Gfp

I discovered the green fluorescent protein GFP from the jellyfish Aequorea aequorea in 1961 as a byproduct of the Ca-sensitive photoprotein aequorin (Shimomura et al., 1962; Johnson et al., 1962), and identified its chromophore in 1979 (Shimomura, 1979). GFP was a beautiful protein but it remained useless for the next 30 years after the discovery. My story begins in 1945, the year the city of N...

Visualization and Functional Analysis of a Maxi-K Channel (mSlo) Fused to Green Fluorescent Protein (GFP)

We have constructed a fusion protein between mSlo (a recombinant, high conductance, calcium-activated potassium channel or maxi-K), and GFP (green fluorescent protein). This construct represents a tag to not only monitor channel expression, but to locate the protein in living cells. The GFP was fused in frame to the carboxy-terminus of the mSlo core protein (mSloGFP fusion protein). Expression ...

Green fluorescent protein (GFP): applications, structure, and related photophysical behavior.

Discovery of green fluorescent protein (GFP) (Nobel Lecture).