Extraction, Enrichment, Solubilization, and Digestion Techniques for Membrane Proteomics

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Extraction/Fractionation Techniques for Proteins and Peptides and Protein Digestion

The use of proteomics approaches is a powerful tool in food science in terms of process optimization and monitoring, quality, traceability, safety, and nutritional assessment (Pedreschi et al. 2010 ) . Proteins, together with peptides, are one of the major groups of food components, and they are found in many different organisms of both vegetal and animal origin. Peptides are also obtained duri...

متن کامل

Development of Techniques for Plasma Membrane and Cell Surface Enrichment

Title of Thesis: Development of Techniques for Plasma Membrane and Cell Surface Enrichment Rebecca L. Rose, Master of Science, 2014 Directed By: Catherine Fenselau, Professor, Department of Chemistry and Biochemistry The goal of this work is to develop and compare two methods of cell surface protein enrichment. The plasma membrane and cell surface proteomes are of great interest for the develop...

متن کامل

Membrane proteomic analysis of Arabidopsis thaliana using alternative solubilization techniques.

This study presents a comparative proteomic analysis of the membrane subproteome of whole Arabidopsis seedlings using 2% Brij-58 or 60% methanol to enrich and solubilize membrane proteins for strong cation exchange fractionation and reversed-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 441 proteins were identified by our Brij-58 method, and 300 proteins were detec...

متن کامل

Optimum conditions for protein extraction from tuna processing by-products using isoelectric solubilization and precipitation processes

The by-product from tuna processing is a potential source of edible protein. Therefore, it is very important to extract protein from such raw materials for human food. In this study the optimum pH for protein extraction from tuna by-products was optimized by using isoelectric solubilization and precipitation processes. The Response Surface Methodology (RSM) and the single factor model were used...

متن کامل

Automating Protein Digestion for Reproducible Proteomics

There are many steps in the process to generating high quality proteomics data on biological samples. There are now powerful MS workflows for quantitation (MRM and SWATH ® acquisition) that enable highly reproducible quantitation on small or large numbers of samples. This creates a new bottleneck in sample preparation, the ability to reproducibly generate digested proteomic samples is critical ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Proteome Research

سال: 2016

ISSN: 1535-3893,1535-3907

DOI: 10.1021/acs.jproteome.5b01122