Filter-based method for background removal in high-sensitivity wide-field-surface-enhanced Raman scattering imagingin vivo
نویسندگان
چکیده
منابع مشابه
Filter-based method for background removal in high-sensitivity wide-field-surface-enhanced Raman scattering imaging in vivo.
As molecular imaging moves towards lower detection limits, the elimination of endogenous background signals becomes imperative. We present a facile background-suppression technique that specifically segregates the signal from surface-enhanced Raman scattering (SERS)-active nanoparticles (NPs) from the tissue autofluorescence background in vivo. SERS NPs have extremely narrow spectral peaks that...
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© 2007 American Institute of Physics, S-0031-9228-0711-020-6 When light interacts with matter, it can scatter inelastically from vibrational quantum states. During that process, photons may lose energy to, or gain it from, vibrational excitations. A change in the photon energy must produce a concomitant shift in the frequency of the scattered light (see box 1). The phenomenon, called the Raman ...
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In recent years numerous studies have shown the potential advantages of molecular imaging in vitro and in vivo using contrast agents based on surface enhanced Raman scattering (SERS), however the low throughput of traditional point-scanned imaging methodologies have limited their use in biological imaging. In this work we demonstrate that direct widefield Raman imaging based on a tunable filter...
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ژورنال
عنوان ژورنال: Journal of Biomedical Optics
سال: 2012
ISSN: 1083-3668
DOI: 10.1117/1.jbo.17.7.076017