Flavonol-Glycoside and Rare Triterpenoid Derivatives Isolated from Leaves of Combretum glutinosum Perr. Ex Dc. with In Vitro Cytotoxic Activity

Combretaceae plants are used traditionally by many cultures, especially in Sudanese patients for the treatment of diverse ailments such as anti-inflammatory, antimicrobial, antitumor, and antioxidant disorders. Of these plants, genus Combretum traditional medicinal plants. Thus, they formed from non-polar or polar extracts isolated phytochemicals. necessities, use their properties can be found earliest myths traditions to document plants’ ability treat diseases. glutinosum Perr. Ex Dc. is a common shrub native African continent, Sudan. Currently, there no published data regarding its cytotoxic activity. Additionally, few chemical biological reports C. glutinosum. Therefore, current study aimed isolate bioactive compounds (1–6) ethyl acetate (EtOAc) extract A new flavonoid compound, namely, glutosinumoside (4), was afforded, five known were obtained: three oleanane-glycosides (1–3) two phenolic acids (5,6). The structures six determined spectroscopic analysis, including one- two-dimensional (1D 2D) NMR, mass spectrometry, chromatographic analysis. Moreover, an vitro evaluation successive EtOAc fractions against MCF7 (breast), HT29 (colon), HepG2 (liver), MRC5 (normal lung) cell lines performed. showed comparable activities with crude EtOH doxorubicin tested lines. Compounds (1) (6) exhibited highest cytotoxicity (1.37 ± 0.21 1.48 0.34 µg/mL, respectively) (3.30 0.02 2.10 0.22 MTT assay. In addition, (3) demonstrated significant upregulation cancer’s important hallmarks (caspase 3 bax genes) inducing apoptosis perturbing cycle.