Flow Cytometric Human Leukocyte Antigen-B27 Typing with Stored Samples for Batch Testing
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چکیده
منابع مشابه
Flow Cytometric Human Leukocyte Antigen-B27 Typing with Stored Samples for Batch Testing
BACKGROUND Flow cytometry (FC) HLA-B27 typing is still used extensively for the diagnosis of spondyloarthropathies. If patient blood samples are stored for a prolonged duration, this testing can be performed in a batch manner, and in-house cellular controls could easily be procured. In this study, we investigated various methods of storing patient blood samples. METHODS WE COMPARED FOUR STORA...
متن کاملHuman Leukocyte Antigen B27 in 453 Asian Indian Patients with Seronegative Spondyloarthropathy
Background: Spondyloarthropathies are a group of closely related inflammatory arthritis which involve the axial skeleton and are negative for rheumatoid factor. Objective: This case-control study was conducted to examine HLA- B27 positivity in patients with seronegative spondyloarthritis (SSA) as per ESSG criteria and compare the frequency with healthy controls because a lower positivity is rep...
متن کاملHuman Leukocyte Antigen-B27 and Disease Susceptibility in Vojvodina, Serbia
There are numerous studies showing the role of human leukocyte antigens (HLAs) related with susceptibility or resistance to certain diseases. The aim of this study was to determine the association of HLA-B27 with ankylosing spondylitis (AS), polyarthralgia, lumboishialgia, acute anterior uveitis (AAU), psoriatic arthritis (PA), synovitis coxae and rheumatoid arthritis (RA) in patients from Vojv...
متن کاملLong-term stabilized blood samples as controls for flow cytometric HLA-B27 screening: a feasibility study.
BACKGROUND Long-term stabilized blood samples are potentially useful as positive or negative procedure controls for flow cytometric HLA-B27 screening, and could serve as test samples in an external quality assessment (EQA) scheme. We evaluated long-term stabilized whole blood specimens as prepared for the UK NEQAS for Leucocyte Immunophenotyping EQA scheme (Sheffield, UK). METHODS Peripheral ...
متن کاملCOMPARISON OF VAL IDITY OF MICROLYMPHOCYTOTOXICITY AND FLOWCYTOMETRY METHODS WITH PCR FOR HL A-B27 ANTIGEN TYPING
The Human Major Histocompatibility Complex (MHC) plays a crucial role in transplantation, transfusion, paternity test and assessment of susceptibility to some diseases associated with HLA-B27. Three of the most fashionable methods for determination of HLA antigens in clinical and research laboratories are microlymphocytotoxicity (MLCT), flowcytometry and polymerase chain reaction (PCR). T...
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ژورنال
عنوان ژورنال: Annals of Laboratory Medicine
سال: 2013
ISSN: 2234-3806,2234-3814
DOI: 10.3343/alm.2013.33.3.174