Flow DNA analysis of primary bone tumors. Relationship between cellular dna content and histopathologic classification
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منابع مشابه
Flow Cytometric DNA Analysis and Histopathologic Re-Evaluation of Paraffin Embedded Samples from Hydatidiform Moles and Hydropic Abortions
Background Distinction of hydatidiform moles (HMs) from non-molar abortions and sub-classification of HMs are important for clinical practice; yet, diagnosis based solely on morphology is affected by interobserver variability. The objective of this study was to determine the role of DNA flow cytometry in distinguishing molar from non-molar pregnancies. MaterialsAndMethods This retrospective stu...
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To investigate the patterns of DNA ploidy and proliferating activity in breast cancer and relate them to other prognostic factors, paraffin blocks of 53 cases of breast carcinoma were studied. Cancer cells obtained by mechanical tissue disaggregation were examined for DNA content, ploidy and S-phase fraction. DNA assay was done using a CAS interactive image analyzing system. All of the cas...
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DNA abundance provides important information about cell physiology and proliferation activity. In a typical in vitro cellular assay, the distribution of the DNA content within a sample is comprised of cell debris, G0/G1-, S-, and G2/M-phase cells. In some circumstances, there may be a collection of cells that contain more than two copies of DNA. The primary focus of DNA content analysis is to d...
متن کاملComparison of image analysis and flow cytometric determination of cellular DNA content.
A good correlation (r = 0.94) was obtained between the DNA indices (DI) using flow cytometry and image analysis of nuclei cytospins extracted from paraffin wax embedded tumour sections. Some of the limitations and problems associated with image analysis which came to light included an unacceptably high coefficient of variation (CV) and a "left-shift" in the DI in most DNA histograms obtained wh...
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ژورنال
عنوان ژورنال: Cancer
سال: 1984
ISSN: 0008-543X,1097-0142
DOI: 10.1002/1097-0142(19840101)53:1<129::aid-cncr2820530123>3.0.co;2-o