Fluorescence-based method to assess the activity of chaperone-mediated autophagy
نویسندگان
چکیده
منابع مشابه
Correction: Establishment of a Novel Fluorescence-Based Method to Evaluate Chaperone-Mediated Autophagy in a Single Neuron
BACKGROUND Chaperone-mediated autophagy (CMA) is a selective autophagy-lysosome protein degradation pathway. The role of CMA in normal neuronal functions and in neural disease pathogenesis remains unclear, in part because there is no available method to monitor CMA activity at the single-cell level. METHODOLOGY/PRINCIPAL FINDINGS We sought to establish a single-cell monitoring method by visua...
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www.sciencemag.org (this information is current as of January 22, 2009 ): The following resources related to this article are available online at http://www.sciencemag.org/cgi/content/full/323/5910/124 version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag.org/cgi/content/full/323/5910/124/DC1 can be foun...
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Intracellular protein degradation rates decrease with age in many tissues and organs. In cultured cells, chaperone-mediated autophagy, which is responsible for the selective degradation of cytosolic proteins in lysosomes, decreases with age. In this work we use lysosomes isolated from rat liver to analyze age-related changes in the levels and activities of the main components of chaperone-media...
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autophagy at a glance Susmita Kaushik1, Urmi Bandyopadhyay1,*, Sunandini Sridhar1, Roberta Kiffin1, Marta Martinez-Vicente1,‡, Maria Kon1, Samantha J. Orenstein1, Esther Wong1 and Ana Maria Cuervo1,§ 1Department of Developmental and Molecular Biology and Institute for Aging Studies, Albert Einstein College of Medicine, Bronx, NY 10461, USA *Present address: Department of Genetics, Yale Universi...
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ژورنال
عنوان ژورنال: Folia Pharmacologica Japonica
سال: 2015
ISSN: 0015-5691,1347-8397
DOI: 10.1254/fpj.145.206