FLUORESCENT DYE METHOD FOR DETERMINATION OF BILIRUBIN-BINDING CAPACITY OF SERUM ALBUMIN
نویسندگان
چکیده
منابع مشابه
Evaluation of a Dye-binding Method for Determination of Serum Albumin.
A simple dye-binding method for determining serum albumin, which employs the dye, 2-(4’-hydroxybenzeneazo) benzoic acid (HBABA), is described. Albumin in 0.1 ml. of serum is determined colorimetrically at 510 m s. Reliability of the method has been established by comparison with another method using HBABA and with a salt fractionation-biuret method, by recovery experiments, variation in ambient...
متن کاملA more accurate dye-binding method for the routine determination of serum albumin.
where U denotes the upper limit value. These two equations were used to construct the respective regression lines in Figure 1. With reference to this figure, observed ranges are classified in relation to the upper limit values appropriate for each duplicate mean. Figure 1 indicates that the acceptable range of duplicates at the high percent of trace binding portion of the calibration curve is a...
متن کاملEffects of serum-isolated vs synthetic bilirubin-albumin complexes on dye-binding methods for estimating serum albumin.
Bush and Reed reported (Clin Chem 1987;33:821-3) that the reaction of albumin with bromcresol purple but not with bromcresol green underestimated the concentration of albumin in synthetically obtained bilirubin-albumin (Bd) by 29%. Their unproven assumption was that chemically synthesized Bd behaved in a manner indistinguishable from the natural Bd in icteric serum. Here we verify that Bd, whet...
متن کاملBovine Serum Albumin–dye Binding
The interaction of the dye reactive bright red 6C with bovine serum albumin has been investigated. Formation of a complex between reactive bright red 6C and bovine serum albumin was detected by use of absorption difference spectroscopy. The curves obtained by titration of the protein against the dye were recorded at different pH and ionic strength of the solution. An unusual form of titration c...
متن کاملNew automated dye-binding method for serum albumin determination with bromcresol purple.
We describe a new automated dye-binding method for serum albumin determination with bromcresol purple (BCP) that has several advantages over an existing bromcresol green (BCG) method. The continuous-flow method is sensitive, linear, and precise, with negligible sample interaction at an analytical rate of 60 samples per hour. Unlike BCG, BCP did not react with an albumin-free serum globulin prep...
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ژورنال
عنوان ژورنال: Pediatric Research
سال: 1974
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-197404000-00385