Formulation optimization and antioxidant test for Self-nano emulsifying drug delivery system of soursop leaves (Annona muricata L.) chloroform extract using candlenut oil as oil phase

Soursop leaves ( Annona muricata L.) chloroform extract has an anticancer agent that can be developed for traditional medicine preparation, even though the extract's solubility is low in water. This study aims to generate new soursop Self-Nano Emulsifying Drug Delivery System (SNEDDS). The expected result increases so drug delivery system more effective. extraction process of uses percolation method with as a solvent. It used tween 80 and cremophore E.L. surfactant, propylene glycol cosurfactant, candlenut oil carrier, make SNEDDS formulation. Then performed comparison between optimal parameters physical properties formulation predictive Simplex Lattice Design (SLD) students' t-test statistical analysis [p> 0.05]. carried out several tests such loading dosage extractability, accelerated stability test, particle size, zeta potential. Furthermore, antioxidant test was using DPPH reagent. optimum composition oil, glycol, mixture 80, cremophor based on SLD 12%: 22%: 66%, respectively. produces stable nanoemulsion, homogenous. transmittance value 90.58% ± 0.151, emulsification time 59.44 1.763 seconds, separation phase 1. results showed no significant difference observations design expert software. produce 25.0 mg size 411.4 nm, polydispersion index 0.482, potential 34.2 mV. IC50 36.28 ppm indicated high.