Frequency of BCoV detection by a semi-nested PCR assay in faeces of calves from Brazilian cattle herds
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چکیده
منابع مشابه
Detection of Bovine Viral Diarrhea Virus Using a Nested RT-PCR Assay in Bulk Milk Samples of Dairy Cattle Herds in Suburb of Mashhad-Iran
Bovine viral diarrhoea virus (BVDV) is an important pathogen of dairy cattle. In this study, bulk milk samples representing a total of 4105 milking cows, from 18 dairy cattle herds in the suburb of Mashhad- Iran, were tested for presence of BVDV by the use of a nested reverse transcription polymerase chain reaction (Nested RT- PCR) assay. Non of the cows in the herds had been vaccinated against...
متن کاملDetection of fungi by conventional methods and semi-nested PCR in patients with presumed fungal keratitis
Background and Purpose: Fungal keratitis is a suppurative, ulcerative, and sight-threatening infection of the cornea that sometimes leads to blindness. The aims of this study were: recuperating facilities for laboratory diagnosis, determining the causative microorganisms, and comparing conventional laboratory diagnostic tools and semi-nested PCR. Materials and Methods: Sampling was conducted in...
متن کاملdetection of bovine viral diarrhea virus using a nested rt-pcr assay in bulk milk samples of dairy cattle herds in suburb of mashhad-iran
bovine viral diarrhoea virus (bvdv) is an important pathogen of dairy cattle. in this study, bulk milk samples representing a total of 4105 milking cows, from 18 dairy cattle herds in the suburb of mashhad- iran, were tested for presence of bvdv by the use of a nested reverse transcription polymerase chain reaction (nested rt- pcr) assay. non of the cows in the herds had been vaccinated against...
متن کاملDetection of Colicin genes by PCR in Escherichia coli isolated from cattle in Shiraz-Iran
A variety of probiotic bacteria have been tested to control animal and foodborne pathogenic bacteria in livestock. The mechanism of inhibition of pathogenic bacteria for several of those probiotic microorganisms is mediated by the production of bacteriocins. Colicins are probably the group of bacteriocins that have been most thoroughly characterized. Colicins are antimicrobial proteins produced...
متن کاملA semi-nested PCR assay for molecular detection of Paracoccidioides brasiliensis in tissue samples.
INTRODUCTION Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis. METHODS In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction, while the primers MJ03 and ITS1 primer were used in the second reaction. The semi-nested PCR was used to investigate biopsies of five patients...
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ژورنال
عنوان ژورنال: Tropical Animal Health and Production
سال: 2009
ISSN: 0049-4747,1573-7438
DOI: 10.1007/s11250-009-9347-2