From directed evolution to computational enzyme engineering—A review
نویسندگان
چکیده
منابع مشابه
Directed evolution of an RNA enzyme.
An in vitro evolution procedure was used to obtain RNA enzymes with a particular catalytic function. A population of 10(13) variants of the Tetrahymena ribozyme, a group I ribozyme that catalyzes sequence-specific cleavage of RNA via a phosphoester transfer mechanism, was generated. This enzyme has a limited ability to cleave DNA under conditions of high temperature or high MgCl2 concentration,...
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Enzyme engineering is undergoing the most profound and exciting transformation in its history (1). It promises unprecedented expansion in the scope and applications of modified or improved enzymes with desired physical and catalytic properties. Two complementary strategies are currently available: rational redesign (2-5) and directed evolution (6,7). Although both approaches have met with great...
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Michael J. McLachlan, Ryan P. Sullivan and Huimin Zhao Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA Departments of Chemical and Biomolecular Engineering, and Chemistry, University of Illinois at Urbana-Champa...
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In this review, we will explore recent computational approaches to understand enzyme evolution from the perspective of protein structure, dynamics and promiscuity. We will present quantitative methods to measure the size of evolutionary steps within a structural domain, allowing the correlation between change in substrate and domain structure to be assessed, and giving insights into the evolvab...
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ژورنال
عنوان ژورنال: AIChE Journal
سال: 2019
ISSN: 0001-1541,1547-5905
DOI: 10.1002/aic.16847