Functional Analysis of PsARRO−1 in Root Development of Paeonia suffruticosa

The adventitious rooting−related oxygenase (ARRO−1) gene is specifically expressed in the early rooting stage and considered a molecular marker of rooting. In this study, PsARRO−1 (GenBank accession number KJ620008) was identified from pre−constructed transcriptome database root development Paeonia suffruticosa under sandy loam cultivation. expression verified by RT−qPCR, it found that trend consistent with database. highly during primordium germination phase. addition, RT−qPCR analysis indicated roots significantly higher than stems leaves, its peak vitro 5 days earlier soil, tree peony soil. Subcellular localization showed localized plasma membrane. Moreover, transient silent seedlings grown using VIGS technology. activity reduced after silencing gene. These results indicate has positive regulatory effect on development.