Functional TRPV1 expression in human corneal fibroblasts

Dynamic studies of human corneal fibroblasts.

Keratocytes ordinarily exist in a quiescent state. However, following injury or after surgery they become activated and fibroblastic and drive the repair of the corneal matrix, a process that is highly dynamic and occurs in three dimensions (3D). Previously, MironMendoza and associates used time-lapse microscopy to study the migration of human corneal fibroblasts out of compressed extracellular...

Functional gap junctions in corneal fibroblasts and myofibroblasts.

PURPOSE Within the corneal stroma, keratocytes communicate through gap junctions. These plasma membrane channels, which connect the cytoplasm of adjacent cells, are composed of connexins. In a cell culture model, an investigation was conducted to determine whether connexin-based gap junction intercellular communication is present in fibroblasts and myofibroblasts, both of which replace keratocy...

Inhibition of poly(I:C)–induced matrix metalloproteinase expression in human corneal fibroblasts by triptolide

PURPOSE Triptolide is a major component of the herb Tripterygium wilfordii Hook f, extracts of which are used in traditional Chinese medicine, and it has been found to possess immunosuppressive and anti-inflammatory properties. Viral infection of the cornea can lead to corneal ulceration and perforation as a result of collagen degradation in the corneal stroma. We have now examined the effect o...

Gene expression and miR profiles of human corneal fibroblasts in response to dexamethasone.

PURPOSE Dexamethasone (DEX) is commonly used as a therapeutic agent for various ocular inflammatory diseases; however, its effect on resident naive cells is unknown. In this study, genome microarray and microRNA (miR) analyses were used to evaluate the global gene and miR expression of human corneal fibroblasts (HKFs) in response to treatment with DEX. METHODS Primary HKFs from three donors w...

Activation in Human Corneal Fibroblasts Adenovirus Infection Is Mediated by c-Src Corneal IL-8 Expression Following