Further characterization of the polynucleotide phosphorylase of Micrococcus luteus
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چکیده
منابع مشابه
A deoxyadenylate kinase activity associated with polynucleotide phosphorylase from Micrococcus luteus.
We report here the presence of two enzymatic activities associated with highly purified preparations of polynucleotide phosphorylase from Micrococcus luteus. The first, a nuclease activity, which is not separated from the phosphorylase on hydroxylapatite, may be due to substitution of H2O for phosphate in the phosphorolysis reaction. The second activity, a deoxyadenylate kinase, the bulk of whi...
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The enzyme, polynucleotide phosphorylase, catalyzes the polymerization of nucleoside diphosphates to polyribonucleotides with the formation of inorganic orthophosphate (1, 2). The reaction is readily reversible, and the phosphorolysis of polyribonucleotides has been studied extensively (3-6). Several recent reviews (7-9) afford extensive summaries of the literature. In the accompanying paper (l...
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An w protein capable of a concerted breaking and rejoining of DNA backbone bonds has been purified from Micrococcus luteus to homogeneity. The protein is a single polypeptide with a molecular weight of 120,000. It catalyzes the removal of superhelical turns from a highly negatively twisted DNA efficiently. The reaction requires Mg2+. In a medium containing 0.1 M K+, the enzyme acts in distribut...
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Three polynucleotide phosphorylase mutations, isolated in heavily mutagenized Escherichia coli strains Q7, Q13, and Q27, were characterized after their transfer by P1 transduction to nearly isogenic strains which lack ribonuclease I. Each strain has a different altered form of polynucleotide phosphorylase. One enzyme exhibited sharply reduced activity under all conditions tested. A second had r...
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A simple procedure for purifying polynucleotide phosphorylase from Escherichin coli cells by means of affinity chromatography on an RNA-Sepharose column is described. The purified enzyme preparation has a specific activity 3500-fold that of the crude extract and is essentially homogeneous, as determined by ultracentrifugation, polyacrylamide gel electrophoresis under denaturing conditions, isoe...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1975
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/2.2.149