G Protein Subunit Dissociation and Translocation Regulate Cellular Response to Receptor Stimulation
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چکیده
منابع مشابه
G Protein Subunit Dissociation and Translocation Regulate Cellular Response to Receptor Stimulation
We examined the role of G proteins in modulating the response of living cells to receptor activation. The response of an effector, phospholipase C-beta to M3 muscarinic receptor activation was measured using sensors that detect the generation of inositol triphosphate or diacylglycerol. The recently discovered translocation of G betagamma from plasma membrane to endomembranes on receptor activat...
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A growing body of evidence suggests that GPCRs exist and function as dimers or higher oligomers. The evidence for GPCR dimerization comes from biochemical, biophysical and functional studies. In addition, researchers have shown the occurrence of heterodimerization between different members of the GPCR family. Two receptors can interact with each other to make a dimer through their extracellular...
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Mechanisms for heterotrimeric G protein activation that do not rely on G protein coupled receptor activation are becoming increasingly apparent. We recently identified beta gamma subunit-binding peptides that we proposed bound to a "hot spot" on beta gamma subunits, stimulating G protein dissociation without stimulating nucleotide exchange and activating G protein signaling in intact cells. AGS...
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The IL-8 (CXCL8) receptors CXCR1 and CXCR2 couple to Gαi to induce leukocyte recruitment and activation at sites of inflammation. We recently showed that CXCR1 couples predominantly to the G protein-coupled receptor kinase (GRK)2, whereas CXCR2 interacts with GRK6 to regulate cellular responses. In addition to G protein-coupled receptors, GRKs displayed a more diverse protein/protein interactio...
متن کاملG-protein subunit dissociation is not an integral part of G-protein action.
The concept that a guanosine triphosphate (GTP) binding protein (or G protein) is a transducer of receptor-to-effector signal transduction was formulated in the 1970s for hormonedependent adenylyl cyclase. It has been shown that binding of the hormone to the receptor triggers exchange of guanosine diphosphate (GDP) for GTP on the G protein, thereby converting the G protein from the inactive con...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2009
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0007797