Gas Liquid Chromatography of Trichothecene Mycotoxins

A simple method with liquid chromatography/tandem mass spectrometry for the determination of the six trichothecene mycotoxins in rice medium.

A selective and speedy LC-MS/MS method was developed to determine six trichothecene mycotoxins (nivalenol, deoxynivalenol, fusarenon X, 15-acetyldeoxynivalenol, 3-acetyldeoxynivalenol, and T-2 toxin) in rice medium where Fusarium graminearum were cultivated for in vitro tests. The analytes were extracted from the rice medium with acetonitrile/water (85/15, v/v), and diluted with acetonitrile/wa...

A Method Using Gas Chromatography – Mass Spectrometry for the Detection of Mycotoxins from Trichothecene Groups A and B in Grains

Mycotoxins are toxic secondary metabolites produced by several fungi species growing on many agricultural commodities and processed foods (Bennett & Klich, 2003). The term secondary metabolite designates compounds that are not indispensable for the growth and metabolism of the fungi as such, and in contrast to primary metabolites, such as amino acids, fatty acids, saccharides, nucleic acids and...

Gas and Liquid Chromatography

Trichothecene mycotoxins from Fusarium culmorum cultures.

Chemical analysis of the culture filtrates of Fusarium culmorum CMI 14764 has demonstrated the presence of seven trichothecene mycotoxins. Major metabolites are 3-acetyldeoxynivalenol and 7 alpha, 8 alpha-dihydroxycalonectrin, with 3,15-diacetyldeoxynivalenol, deoxynivalenol, calonectrin, isotrichodermin and 12,13-epoxytrichothec-9-ene (EPT) as minor products. The occurrence of the rarely encou...

Wine: Gas and Liquid Chromatography

From the chemical point of view, wines are aqueous alcoholic solutions containing more than 1000 components that can be present at high concentrations (g L ), but also at trace levels (ng L ). Some of these components determine the organoleptic properties of wines, while others are signi"cant for classifying their origin and/or for checking whether some adulteration has taken place. Concentrati...