Gene expression level: is it an important factor in codon optimization for overexpression of recombinant proteins?
نویسندگان
چکیده
منابع مشابه
Gene expression level: is it an important factor in codon optimization for overexpression of recombinant proteins?
Background Microalgae are becoming a viable model for the expression of recombinant proteins. However, suitable levels of expression for commercial production have not yet been obtained. One way to increase the heterologous protein production is the codon optimization of the target gene so that it presents the preferential codons used by the host organism according to its own pool of transfer R...
متن کاملUtility of P19 Gene-Silencing Suppressor for High Level Expression of Recombinant Human Therapeutic Proteins in Plant Cells
Background: The potential of plants, as a safe and eukaryotic system, is considered in the production of recombinant therapeutic human protein today; but the expression level of heterologous proteins is limited by the post-transcriptional gene silencing (PTGS) response in this new technology. The use of viral suppressors of gene silencing can prevent PTGS and improve transient expression level ...
متن کاملCodon optimization and cloning of bovine prochymosin gene for proper expression in tobacco plant
Bovine chymosin enzyme is one of the most commonly used enzymes in the dairy industry. The production of this enzyme from its natural source does not meet the needs of this huge industry. The production of recombinant bovine chymosin in plants can be a good alternative to native enzyme. Insertion and expression of foreign genes in plants can occur in the nucleus and chloroplast organelles. The ...
متن کاملCodon Optimization, Cloning and Expression of the Human Leukemia Inhibitory Factor (hLIF) in E. coli
Background: Leukemia inhibitor factor (LIF) is a very important pleiotropic cytokine which belongs to interleukin-6 (IL-6) family. LIF exerts multiple effects on different types of cells and tissues with numerous regulatory effects in vivo and in vitro. It is a lymphoid factor, which performs a number of activities including cholinergic neuron differentiation, contro...
متن کاملCloning, Codon Optimization, and Expression of Yersinia intermedia Phytase Gene in E. coli
Background: Phytate is an anti-nutritional factor in plants, which catches the most phosphorus contents and some vital minerals. Therefore, Phytase is added mainly as an additive to the monogastric animals’ foods to hydrolyze phytate and increase absorption of phosphorus. Objectives: Y. intermedia phytase is a new phytase with special characteristics such as high specific activity, pH stabilit...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Proceedings
سال: 2014
ISSN: 1753-6561
DOI: 10.1186/1753-6561-8-s4-p224