Genetic Diversity Assessment in Pea (Pisum sativum L.) using Microsatellite Markers

The present study was conducted on genetic diversity analyses among 24 pea genotypes during 2017–2018 to assess the molecular of using SSR markers. Out 62, eleven markers were found be polymorphic and information content (PIC) simple sequence repeat (SSR) ranged from 0.19 as high 0.64. Molecular profiling these 11 SSRs distributed throughout genome generated 32 alleles with a mean 2.91 per locus. dissimilarity based matching coefficient for 0.00 0.91 an average 0.52. Cluster grouped into two major clusters one outlier supported by principal coordinate analysis (PCoA) in which across four quadrangles. Analysis variance (AMOVA) showed significant estimated value at degree 1000 permutations. Percentage variability higher individual (67%) than populations (11%). within also (22%) Pop1 (I=0.707, He=0.446, uHe=0.466) shows pop2 (I=0.630, He=0.381 uHe=0.398). percentage loci population (PPL) 81.82% (pop2) 90.91% (pop1) 86.36%. demonstrates utility microsatellite estimating well genotype identification pea. This suggests potential use further association studies.