Genetic transformation of apical meristematic shoots in the banana cultivar ‘Williams’

Bananas and plantains (Musa spp.) are among the most critical socioeconomic crops globally, being a staple food for millions of people in tropics an essential component export market, including subtropics. Besides conventional breeding, genetic improvement bananas could be performed through engineering new breeding techniques. Furthermore, plant tissue culture is these technologies, developing embryogenic cell suspensions vitro plants. The transient stable transformation from plants, shortening Musa transgenic lines development compared to while using suspension. In this study, protocol was established banana apical meristems ‘Williams’ cultivar (genotype AAA). based on co-cultivation explants (whole plants or bisected meristematic tissues derived plants) with Agrobacterium tumefaciens strain LBA4404 harboring two binary vectors denominated pLVCIBE1 (cassette: MabHIPP promoter::luc2::Tnos, P35S::hpt::Tnos) pLVCIBE2 P35S::luc2::Tnos, P35S::hpt::Tnos), independently. obtained by subculturing selection medium (12.5µg/mL hygromycin) 8 weeks transformation. Genetic confirmed vivo use luciferase reporter gene system. PCR DNA extracted leaves regenerated after selection, confirming Therefore, achieved tumefaciens.