Genome-Wide Identification and Expression Analysis of Cytosine DNA Methyltransferase Genes Related to Somaclonal Variation in Pineapple (Ananas comosus L.)

DNA methylation plays an important role in somaclonal variation. Cytosine-5 methyltransferases (C5-MTases) are crucial enzymes for maintaining the de novo genome status, and gene family has been identified characterized several species. However, limited information is available about of C5-MTases variation pineapple. Herein, characteristic leaf variants pineapple showed that rate decreased demethylation was mainly. Six AcC5-MTases were from genome. Phylogenetic analysis indicated six members could be classified into four subgroups, namely, AcMET, AcCMT, AcDRM, AcDNMT, demonstrating evolutionary conservation across other plant Cis-elements presented AcC5-MTase promoters, including light-responsive, phytohormone-responsive, growth development elements. Furthermore, we investigated expression profiles different tissues their transcript abundance leaves Interestingly, most genes highly expressed carpopodium. AcCMT2, AcDRM2 upregulated variants, indicating might play Our results will aid understanding complex roles plants provide a basis analyzing modification